Ned B. Klopfenstein, Moscow Forestry Sciences Laboratory

Ned B. Klopfenstein
Rocky Mountain Research Station
1221 South Main
Moscow, Idaho 83843 U.S.A.
ph: (208) 883-2310
fax: (208) 883-2318

EDUCATION:
Ph.D. in Plant Pathology, Iowa State University (1985)
B.Sci. in Biology (Chemistry minor), Iowa State University (1976)

PROFESSIONAL EXPERIENCE:
2000-present Research Plant Pathologist, USDA Forest Service, Moscow, ID
1998-2000: Plant Pathologist, USDA Forest Service, Moscow, ID
1999-present: Affiliate Assistant Professor, College Natural Resources, Division of Forest Resources, University of Idaho, Moscow, ID
1990-present: Adjunct Assistant Professor, School of Natural Resource Sciences, University of Nebraska, Lincoln, NE
1990-1998: Research Plant Pathologist, USDA Forest Service, National Agroforestry Center, Lincoln, NE
1985-1990: Postdoctoral Research Assistant/Associate, Depts. of Forestry and Plant Pathology, Iowa State University, Ames, IA
1979-1985: Graduate/Research/Teaching Assistant, Dept. of Plant Pathology, Iowa State University, Ames, IA

RESEARCH INTERESTS:
forest pathology, molecular diagnostics of forest microbes, population genetics of forest hosts and pathogens, phylogenetics of forest pathogens, environmental influences on genetic structure of forest hosts and pathogens, woody plant defense systems, host/parasite interactions, expression of plant defense genes, and plant symbioses.

PROFESSIONAL ORGANIZATIONS:
American Phytopathological Society
Society of American Foresters
Western International Forest Disease Work Conference
American Poplar Council
Native Plant Society
International Union of Forestry Research Organizations working parties (S2.04-07 Somatic Cell Genetics; S2.04-06 Molecular Genetics; 7.02.01 root and butt rots)
Great Plains Tree Pest Council
International Poplar Commission
Southern Nursery Association
Inland Empire Tree Improvement Cooperative
Intermountain Forest Tree Nutrition Cooperative

PUBLICATIONS:

PUBLISHED

(52) McDonald, G.I., P.J. Zambino, N.B. Klopfenstein . 2005. Naturalization of host-dependent microbes after introduction into terrestrial ecosystems: Evolutionary epidemiology of white pine blister rust. Pp. 41-57 in: Lundquist, J.E., and R.C. Hamelin, eds. From molecules to ecosystems - Forest pathology in the era of genes and landscapes. St. Paul , MN : American Phytopathological Society Press. Chapter 5. (Invited).

(51) Richardson, B.A., N.B. Klopfenstein , T.L. Peever. 2005. Assessing forest-pathogen interactions at the population level. Pp. 21- 30 in: Lundquist, J.E., and R.C. Hamelin, eds. From molecules to ecosystem - Forest pathology in the era of genes and landscapes. St. Paul , MN : American Phytopathological Society Press. Chapter 3. In press. (Invited).

(50) Kim, M.-S., N.B. Klopfenstein , and R.C. Hamelin. 2005. Application of molecular genetic tools for the study of forest pathosystems. Pp. 9-20 in: Lundquist, J.E., and R.C. Hamelin, eds. From molecules to ecosystems - Forest pathology in the era of genes and landscapes. St. Paul , MN : American Phytopathological Society Press. Chapter 2. In press. (Invited).

(49) Mmbaga, M.T., Klopfenstein, N.B. , Kim, M.-S. Mmbaga , N.C. 2004. PCR-based identification of Erysiphe pulchra and Phyllactinia guttata from Cornus florida using ITS-specific primers . Forest Pathology. 34: 321-328. (Refereed)

(48) Hoff, J.A., N.B. Klopfenstein , G.I. McDonald, J.R. Tonn, M.-S. Kim, P.J. Zambino, P.F. Hessburg, J.D. Rogers, T.L. Peever, and L.M. Carris. 2004. Fungal endophytes in woody roots of Douglas-fir ( Pseudotsuga menziesii ) and ponderosa pine ( Pinus ponderosa ) . Forest Pathology 34: 255-271. (Refereed)

(47) Hoff, J.A., N.B. Klopfenstein , J.R. Tonn, G.I. McDonald, P.J. Zambino, J.D. Rogers, T.L. Peever, and L.M. Carris. 2004. Roles of woody root-associated fungi in forest ecosystem processes: Recent advances in fungal identification . Res. Pap. RMMR-RP-47. Fort Collins , CO : U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. 6 p.

(46) Kim, M.-S., S.J. Brunsfeld, G.I. McDonald, and N.B. Klopfenstein . 2003. Effect of white pine blister rust ( Pinus monticola Dougl.) and rust-resistance breeding on genetic variation in western white pine ( Pinus monticola ). Theoretical and Applied Genetics 106:1004-1010. (Refereed)

(45) Richardson, B.A., N.B. Klopfenstein , and S.J. Brunsfeld. 2002. Assessing Clark's nutcracker seed-caching flights using maternally inherited mitochondrial DNA of whitebark pine . Canadian Journal of Forest Research 32: 1103-1107 (Refereed).

(44) Richardson, B.A., Brunsfeld, S.J., and N.B. Klopfenstein . 2002 . DNA from bird-dispersed seen and wind disseminated pollen provides insights into postglacial colonization and population genetic structure of whitebark pine ( Pinus albicaulis ). Molecular Ecology 11: 214-227. (Refereed).

(43) Klopfenstein, N.B. , G.I. McDonald, M.-S. Kim, S.J. Brunsfeld, B.A. Richardson, and J. E. Lundquist. 2001. Molecular genetic approaches to risk assessment in forest ecosystems. pp. 108- 121 in: Proceedings of the Society of American Foresters national convention. 16-20 November 2000. Washington , D.C. (Invited).

(42) Kim, M.-S., N.B. Klopfenstein , G.I. McDonald, K. Arumuganathan, and A.K. Vidaver. 2001. Use of flow cytometry, fluorescence microscopy, and PCR-based techniques to assess intraspecific and interspecific mating of Armillaria species . Mycological Research 105(2): 164-172. (Refereed).

(41) Mmbaga, M.T.; Klopfenstein, N.B. ; Kim, M.S. 2000. Molecular genetic analysis of powdery mildew pathogens of dogwood. In : Bryson, J.L., ed. and compiler. Proceedings of the Southern Nursery Association Research Conference. 45: 236-242. (Invited).

(40) Lundquist, J.E., and N.B. Klopfenstein . 2000. Integrating concepts of landscape ecology with the molecular biology of forest pathogens. Forest Ecology and Management 150(3): 213-222 . (Refereed).

(39) Kim, M.-S., N.B. Klopfenstein , G.I. McDonald, K. Arumuganathan, and A.K. Vidaver. 2000. Characterization of North American Armillaria species by nuclear DNA content and RFLP analysis . Mycologia 92(5): 874-883. (Refereed).

(38) Tabor, G.M., T. Kubisiak, N.B. Klopfenstein , R.B. Hall, and H.S. McNabb, Jr. 2000. Bulk segregant analysis identifies molecular markers linked to Melampsora medusae resistance in Populus deltoides . Phytopathology 90(9): 1039-1042. (Refereed).

(37) Klopfenstein, N.B. and M.E. Dix. 2000. IV. Biological control of weeds. A. Dwarf Mistletoes. In : Coulson, J.R., Vail, P.V., Dix, M.E., Nordlund, D.A., and Kauffman, W.C., eds. 110 Years of Biological Control Research and development in the United States Department of Agriculture: 1883-1993. United States Department of Agriculture. Agricultural Research Service. pp. 465-466. (Invited).

(36) Klopfenstein, N.B. , E.G. Kuhlman, C.M. Schumann, and M.E. Dix. 2000. III. Biological control of forest pathogens. A. Forest Diseases. In : Coulson, J.R., Vail, P.V., Dix, M.E., Nordlund, D.A., and Kauffman, W.C., eds. 110 Years of Biological Control Research and development in the United States Department of Agriculture: 1883-1993. United States Department of Agriculture. Agricultural Research Service. pp. 455-458. (Invited).

(35) Dix , M.E. , B. Bishaw, S.W. Workman, M.R. Barnhart, N.B. Klopfenstein , and A.M. Dix. 1999. Pest management in energy- and labor-intensive agroforestry systems. In: Buck, L.E., J.P. Lassoie, and E.C.M. Fernandes, eds. Agroforestry in sustainable agricultural systems. CRC Press, Lewis Publishers, Washington , D.C. Chapter 7. pp. 131-155 (Invited).

(34) Kim, M.-S., N.B. Klopfenstein , and B.M. Cregg. 1998. In vitro and ex vitro rooting of micropropagated shoots using three green ash ( Fraxinus pennsylvanica ) clones. New Forests 16: 43-57. (Refereed).

(33) Klopfenstein, N.B. , Y.W. Chun, M.-S. Kim, and M.R. Ahuja, eds. 1997. Micropropagation, Genetic Engineering, and Molecular Biology of Populus . General Technical Report, RM-GTR-297. Ft. Collins , CO : USDA Forest Service, Rocky Mountain Research Station. 326 p. (34 chapters contributed by 85 international authors).

(32) Dix , M.E. , N.B. Klopfenstein , J.-W. Zhang, S.W. Workman, and M.-S. Kim. 1997. Potential use of Populus for phytoremediation of environmental pollution in riparian zones. In : Klopfenstein, N.B., Y.W. Chun, M.-S. Kim, and M.R. Ahuja, eds. Micropropagation, Genetic Engineering, and Molecular Biology of Populus . General Technical Report, RM-GTR-297. Ft. Collins , CO : USDA Forest Service, Rocky Mountain Research Station. Chapter 27. pp. 206-211 (Invited).

(31) Kim, M.-S., N.B. Klopfenstein , and Y.W. Chun. 1997. Agrobacterium -mediated transformation of Populus . In : Klopfenstein, N.B., Y.W. Chun, M.-S. Kim, and M.R. Ahuja, eds. Micropropagation, Genetic Engineering, and Molecular Biology of Populus . General Technical Report, RM-GTR-297. Ft. Collins , CO : USDA Forest Service, Rocky Mountain Research Station. Chapter 7. pp. 51-59 (Invited).

(30) Heuchelin , S.A. , L. Jouanin, N.B. Klopfenstein , and H.S. McNabb, Jr. 1997. Potential of proteinase inhibitors for enhanced resistance to Populus arthropod and pathogen pests. In : Klopfenstein, N.B., Y.W. Chun, M.-S. Kim, and M.R. Ahuja, eds. Micropropagation, Genetic Engineering, and Molecular Biology of Populus . General Technical Report, RM-GTR-297. Ft. Collins , CO : USDA Forest Service, Rocky Mountain Research Station. Chapter 23. pp. 173-177 (Invited).

(29) Klopfenstein, N.B. , W.J. Reitveld, R.C. Carman, T.R. Clason, S.H. Sharrow, G. Garrett, and B.E. Anderson. 1997. Silvopasture: An agroforestry practice. USDA Forest Service, Rocky Mountain Research Station, National Agroforestry Center , Lincoln , NE. Agroforestry Note - 8. 5 p. (Invited).

(28) Klopfenstein, N.B. and M.-S. Kim. 1997. Molecular genetic approaches to forest ecosystem studies. In : Kim, Y.-K., ed. Proceedings of the International symposium on: Forest science in the 21 st century. 15 October 1997 . Institute of Forest Science, Kookmin University , Seoul , South Korea . pp. 3- 15. (Invited).

(27) Dix , M.E. , E. Akkuza, N.B. Klopfenstein , J. Zhang, M.-S. Kim, and J.E. Foster. 1997. Riparian refugia in agroforestry systems. Journal of Forestry 95(8):38-41. (Refereed).

(26) Heuchelin , S.A. , H.S. McNabb, Jr., and N.B. Klopfenstein . 1997. Agrobacterium -mediated transformation of Populus x euramericana ‘Ogy' using the chimeric CaMV 35S - pin2 gene fusion. Canadian Journal of Forest Research 27:1041-1048. (Refereed).

(25) Zhang, J., N.B. Klopfenstein , and G.W. Peterson. 1997. Genetic variation in disease resistance of Juniperus virginiana and J . scopulorum grown in eastern Nebraska . Silvae Genetica 46(1):11-16. (Refereed).

(24) Klopfenstein, N.B. , K.K. Allen, F.J. Avila, S.A. Heuchelin, J. Martinez, R.C. Carman, R.B. Hall, E.R. Hart, and H.S. McNabb, Jr. 1997. Proteinase inhibitor II gene in transgenic poplar: Chemical and biological assays. Biomass and Bioenergy 12(4):299-311. (Refereed).

(23) Kim, M.-S., C.M. Schumann, and N.B. Klopfenstein . 1997. Effects of thidiazuron and benzyladenine on axillary shoot proliferation of three green ash ( Fraxinus pennsylvanica Marsh.) clones. Plant Cell, Tissue and Organ Culture. 48:45-52. (Refereed).

(22) Klopfenstein, N.B. and E.R. Hart. 1997. Chapter 19: Biotechnological approaches to study and improve insect resistance of woody plants. In : Geneve, R.L., J.E. Preece, and S.A. Merkle, eds. Biotechnology of Ornamental Plants. CAB International, Wallingford , Oxon , UK . pp. 353-366. (Invited).

(21) Dix , M.E. , M. Harrell, N.B. Klopfenstein , K. Barkhouse, R. King, and R. Lawson. 1996. Insect infestations and incidence of western gall rust among ponderosa pine sources grown in the central Great Plains . Environmental Entomology: 25:611-617. (Refereed).

(20) Klopfenstein, N.B. and J.G. Kerl. 1995. Biotechnological contributions to temperate agroforestry practices. Agroforestry Systems 32:29-44. (Refereed).

(19) Avila , F.J., G.Y. Yuen, and N.B. Klopfenstein . 1995. Characterization of a Pythium ultimum -specific antigen and factors that affect its detection using a monoclonal antibody. Phytopathology 85:1378-1387. (Refereed).

(18) Chun, Y.W. and N.B. Klopfenstein . 1995. Organ specific expression of the nos - NPT II gene in transgenic hybrid poplar. Journal of the Korean Forestry Society 84 (1):77-86. (Refereed).

(17) Dix , M.E. , N. Klopfenstein , C. Schumann, B. Cregg, M. Schoeneberger, and M. Dosskey. 1995. Sustainable pest management in agroforestry ecosystems. pp. 198-203 in : Managing Forest to Meet Peoples' Needs. Proceedings of the 1994 Society of American/Canadian Institute of Forestry Convention, Forest Pest Management Working Group Technical Session. 18-22 September 1994, Anchorage , AK . Society of American Foresters, Bethesda , MD.

(16) Dix , M.E. , N. Klopfenstein , W.J. Rietveld, M. Harrell, and J. Walla. 1994. Biodiversity can enhance biological control of pests in Great Plains agroecosystems. pp. 37-53 in : Proceedings of the Great Plains Agricultural Council. 7-9 June 1994. Bismarck , ND . GPAC Publication (Invited).

(15) Dix , M.E. , J. Walla, M. Harrell, N. Klopfenstein , and J. MacFall. 1993. Biological control of forest pests in the Great Plains : status, needs and issues. A white paper prepared by the Forest Biological Control Task Force of the Great Plains Agricultural Council Forestry Committee. 13pp. (derived from Publication No. 13) (Invited).

(14) Cregg, B.M., M.E. Dix, N.B. Klopfenstein , and C.M. Schumann. 1993. Improving stress and pest resistance on trees for agroforestry systems: Challenges and opportunities. pp. 291-297 in : American Forestry--An Evolving Tradition. Proceedings of the Society of American Foresters 1992 National Convention. 25-28 October 1992. Richmond , VA.

(13) Dix , M.E. , J. Walla, M. Harrell, N. Klopfenstein , and J. MacFall. 1993. Biological control of forest pests in the Great Plains : status, needs and issues. pp. 44-52 in : Proceedings of the Biological Control of Forest Pests in the Great Plains : Status and needs - a symposium. Great Plains Agricultural Council, Forestry Committee's Forest Biological Control Task Force. Bismarck , ND , 13-16 June 1992. Great Plains Agricultural Council Publication No. 145. (Invited).

(12) Klopfenstein, N.B. , H.S. McNabb, Jr., E.R. Hart, R.B. Hall, R.D. Hanna, S.A. Heuchelin, K.K. Allen, N.-Q. Shi, and R.W. Thornburg. 1993. Transformation of Populus hybrids to study and improve pest resistance. Silvae Genetica 42:86-90. (Refereed).

(11) Schumann, C.M. and N.B. Klopfenstein . 1992. Contributions of molecular biology to improvement of disease resistance in Great Plains tree species. pp. 102-104 in : Proceedings of the Biological Control of Forest Pests in the Great Plains : Status and needs - a symposium. Great Plains Agricultural Council, Forestry Committee's Forest Biological Control Task Force. Bismarck , ND , 13-16 June 1992. Great Plains Agricultural Council Publication No. 145. (Invited).

(10) Klopfenstein, N.B. , H.S. McNabb, Jr., E.R. Hart, R.B. Hall, R.D. Hanna, S.A. Heuchelin, K.K. Allen, N.-Q. Shi, and R.W. Thornburg. 1992. Molecular approaches toward understanding and improving defense systems of Populus . pp. 79-91 in : Hall, R. B., R. D. Hanna, and R. N. Nyong'o, eds. Joint Proceedings of the International Energy Agencies 1991 Joint Meeting of the Task V Activity Groups on Exchange of Genetic Material; Pest/Disease Management; and Joint Trials of Alnus , Populus , and Salix . 22-27 August 1991. Iowa State University , Ames , IA. (Invited).

(9) Klopfenstein, N.B. , R.W. Thornburg, H.S. McNabb, Jr., R.B. Hall, E.R. Hart, Y.W. Chun, A. Kernan, and N.-Q. Shi. 1991. Transformation of Populus - from system development to field plantings. pp. 357-358 in : M. R. Ahuja, ed. Woody Plant Biotechnology. NATO ASI Series, Series A: Life Sciences Volume 210. Plenum Press, NY.

(8) Klopfenstein, N.B. , N.-Q. Shi, A. Kernan, H.S. McNabb, Jr., R.B. Hall, E.R. Hart, and R.W. Thornburg. 1991. A transgenic Populus hybrid expresses a wound-inducible potato Proteinase Inhibitor II-CAT gene fusion. Canadian Journal of Forest Research 21:1321-1328. (Refereed).

(7) McNabb, H.S., Jr., N.B. Klopfenstein , S.A. Heuchelin, E.R. Hart, R.B. Hall, K.K. Allen, and R.W. Thornburg. 1991. The integration of genetic engineering into Iowa State University 's woody-biomass research program. pp. 66-72 in : Steenackers, V. and P. Smets, eds. Proceeding of the International Energy Agency - Task 5 Activity Groups on Exchange of Genetic Material, Joint Trials of Alnus , Populus and Salix , and Pest/Disease Management. 11-14 September 1990. Geraardsbergen , Belgium . (Invited).

(6) McNabb, H.S., Jr., N.B. Klopfenstein , R.D. Hanna, R.B. Hall, E.R. Hart, S.A. Heuchelin, and R.W. Thornburg. 1990. A field trial of transgenic hybrid poplar trees: Establishment and growth through the second seasons. pp. 155-159 in : MacKenzie, D. R. and S. C. Henry, eds. Biological Monitoring of Genetically Engineered Plants and Microbes. Proceeding of the International Symposium on the Biosafety Results of Field Tests of Genetically Modified Plants and Microorganisms. Kiawah Island , SC , USA . 27-30 November 1990. USDA/CSRS/ARS and Clemson University , Bethesda , MD and Clemson, SC. (Invited).

(5) Ernst, S.E. and N.B. Klopfenstein . 1990. Improvement of woody plant germplasm using a combination of conventional and molecular research techniques. pp. 36-40 in : Trees are the Answer: 42nd Annual Meeting, Forestry Committee, GPAC. 18-21 June 1990. Colorado Springs , CO . Great Plains Agricultural Council Publication 132. (Invited).

(4) Chun, Y.W., N.B. Klopfenstein , H.S. McNabb, Jr., and R. B. Hall. 1988a. Transformation of Populus species by an Agrobacterium binary vector system. J. Korean Forestry Society 77:199-207. (Refereed).

(3) Chun, Y.W., N.B. Klopfenstein , H.S. McNabb, Jr., and R.B. Hall. 1988b. Biotechnological applications in Populus species. J. Korean Forestry Society 77:467-483. (Refereed).

(2) McNabb, H.S., Jr., R.A. Serres, M.M. Cram, and N.B. Klopfenstein . 1986. Biological constraints in the exchange of planting material for energy production. In : Proc. Joint IEA Forestry Energy Programme and FAO/Cooperative Network on Rural Energy Forest Energy Conference and Workshops on Research in Forestry for Energy. Rungstedgaard , Denmark ; 28 October - 1 November 1985 . Swedish University of Agricultural Science. nr. 49/1986. pp. 96-97. (Invited).

(1) Klopfenstein, N.B. 1985. Developmental aspects of Gigaspora rosea and Glomus etunicatum , alone or is association with Alnus glutinosa . Ph.D. dissertation. Iowa State University , Ames , IA. 148p. (Dissertation Abstracts International, section B. 46(05):1408-1409).

IN PREPARATION

(A) Hudgins, J.W., G.I. McDonald, P.J. Zambino, N.B. Klopfenstein, and V.R. Franceschi. Anatomical and cellular responses of western white pine ( Pinus monticola Dougl.) stem tissues to invasion by Cronartium ribicola J.C. Fisch. Submitted to Forest Pathology.

Rocky Mountain Research Station - Moscow Forestry Sciences Laboratory